My view of that moment was that he was frustrated by his body simply not being there as expected leaving him without hope. If you've ever faced such a moment, like when age catches up to you, it can be very hard to accept and feels like betrayal. Real or not, that's what I saw.
Disclosure: I am a tennis fan foremost, and feel that Novak has earned more respect and better treatment than he gets. Thus, I am a Novak fan.